Successful tips of DNA extraction and PCR of plants for,
18-08-2018· The protocol of DNA extraction is simple but consists of many important factors. In the present report we described all imaginable keys for successful DNA extraction from plants, based on our long experience. We would say that DNA can be more or less extracted from any plant species, if the protocols are faithfully followed.CTAB Protocol for the Isolation of DNA from Plant Tissues,Plant samples can be prepared by cryogenically grinding tissue in a mortar and pestle after chilling in liquid nitrogen. Freeze dried plants can be ground at room temperature. In either case, a fine powder is best for extracting DNA. For each 100 mg homogenized tissue use 500 µl of CTAB Extraction Buffer. Mix and thoroughly vortex.High-throughput Tissue Grinder For Nucleic Acid, Protein,,It is a true high-throughput animal and plant tissue grinding machine. Each sample takes only 1-2 minutes to complete. Grinding samples include roots, stems, leaves, flowers, fruits, seeds, and certain animal tissues of plants; particularly suitable for the extraction of nucleic acids, proteins, and other components in plant tissues.